HPLC-UV method setup for hypericin in Cuscuta seed herb
A herbal medicine method for Cuscuta seed targeted hypericin using a C18 column and UV detector, with the 2020 edition of Chinese Pharmacopoeia Part I used as the reference source.
Background
Requirement context
The China site method note gives Tianzhao a concrete pharmaceutical and traditional medicine testing application. It is useful for product pages because it shows how column and detector choices connect to a named compound and reference method.
Selection challenges
What needed confirmation
Use a suitable C18 column for the herbal matrix and target compound.
Confirm UV wavelength and chromatographic conditions before final instrument selection.
Package the column, detector, HPLC system, and method documents for technical review.
Workflow architecture
From requirement to quotation
Instruments
C18 HPLC column, UV detector, HPLC pump/system, injector, and workstation by method requirement.
Confirmation Layer
Target compound, reference method, wavelength, mobile phase, column, and documentation needs.
RFQ Review
Pharmaceutical method RFQ organized around column and detector selection.
Configuration path
Application stages
Column separation
C18 HPLC column
Separate hypericin from the herbal sample matrix.
UV detection
UV detector with HPLC workstation
Detect and review the target compound peak.
Result
RFQ output and buyer value
Reference source
2020 Chinese Pharmacopoeia Part I was listed as the reference.
The application can be presented as a method-driven HPLC configuration.
Method signal
The method note identifies C18 column use and UV detection.
Column and detector requirements should be included in the first RFQ.
The case adds a specific herbal medicine application to the chromatography content.
It supports SEO and buyer review for C18 column, UV detector, and HPLC method selection.
The page can guide buyers to provide target compound, reference method, wavelength, and column needs.
Related products
Instruments referenced in this case
Ultimate C18 Series
C18 HPLC Column
Ultimate C18 HPLC column series for routine reversed-phase liquid chromatography methods requiring stable peak shape, high efficiency, and broad method compatibility.
Workflow fit
Sample injection -> Separation method -> Detector output -> Chromatogram review
RFQ: Target method or analytes
RFQ: Detector and pump configuration
Reversed-phase HPLC analysis / Food and pharmaceutical methods
Details
SPD-500I
SPD-500I Analytical Variable Wavelength UV Detector
Analytical variable wavelength UV detector for HPLC and routine chromatography detection.
Workflow fit
Signal detection -> Wavelength selection -> LC system integration -> Method verification
RFQ: Wavelength range and application
RFQ: Analytical or preparative use
Analytical HPLC detection / QC laboratories
Details
LC500 HPLC
LC500 High Performance Liquid Chromatograph
High performance liquid chromatography system for routine analytical separation, quality control, and laboratory method work.
Workflow fit
Sample injection -> Separation method -> Detector output -> Chromatogram review
RFQ: Target method or analytes
RFQ: Detector and pump configuration
Pharmaceutical quality control / Food and agricultural testing
Details