HPLC method configuration for preservatives and sweeteners in wine
A wine testing workflow used LC500 HPLC, SPD-500 UV detection, manual injection, workstation support, and a C18 column to measure benzoic acid, sorbic acid, acesulfame potassium, and saccharin sodium according to the GB/T 23495-2009 reference method.
Background
Requirement context
The method content from Tianzhao's China site describes a preservative and sweetener test for wine. The application is useful for overseas buyers because it connects the analyte list, reference method, detector, column, and HPLC configuration before quotation.
Selection challenges
What needed confirmation
Confirm the target analytes before selecting detector wavelength, column, and workstation scope.
Match the method to a C18 column and UV detection workflow.
Prepare an RFQ that separates the HPLC system, UV detector, injector, workstation, and column requirements.
Workflow architecture
From requirement to quotation
Instruments
LC500 HPLC system, SPD-500 UV detector, manual injection valve, workstation, and C18 column.
Confirmation Layer
Method requirements covering analytes, reference method, wavelength, column, and data review.
RFQ Review
Food testing RFQ built around complete HPLC method configuration.
Configuration path
Application stages
Separation system
LC500 HPLC with C18 column
Separate wine preservatives and sweeteners before UV detection.
Detection and data review
SPD-500 UV detector, manual injector, and chromatography workstation
Measure benzoic acid, sorbic acid, acesulfame potassium, and saccharin sodium.
Result
RFQ output and buyer value
Reference method
GB/T 23495-2009 was used as the method reference.
The RFQ can be organized around a known food testing method instead of a generic HPLC request.
Method configuration
LC500 HPLC, SPD-500 UV detector, C18 column, injector, and workstation were identified as key components.
The buyer can confirm all method-critical modules before pricing.
The method strengthens Tianzhao's food and beverage testing application coverage.
The case creates a practical cross-link between HPLC systems, UV detectors, and C18 columns.
The application is suitable for food testing laboratories that need preservative and sweetener analysis.
Related products
Instruments referenced in this case
LC500 HPLC
LC500 High Performance Liquid Chromatograph
High performance liquid chromatography system for routine analytical separation, quality control, and laboratory method work.
Workflow fit
Sample injection -> Separation method -> Detector output -> Chromatogram review
RFQ: Target method or analytes
RFQ: Detector and pump configuration
Pharmaceutical quality control / Food and agricultural testing
Details
SPD-500I
SPD-500I Analytical Variable Wavelength UV Detector
Analytical variable wavelength UV detector for HPLC and routine chromatography detection.
Workflow fit
Signal detection -> Wavelength selection -> LC system integration -> Method verification
RFQ: Wavelength range and application
RFQ: Analytical or preparative use
Analytical HPLC detection / QC laboratories
Details
Ultimate C18 Series
C18 HPLC Column
Ultimate C18 HPLC column series for routine reversed-phase liquid chromatography methods requiring stable peak shape, high efficiency, and broad method compatibility.
Workflow fit
Sample injection -> Separation method -> Detector output -> Chromatogram review
RFQ: Target method or analytes
RFQ: Detector and pump configuration
Reversed-phase HPLC analysis / Food and pharmaceutical methods
Details